Data-enriched characterization of mobile populations. Morphological information from illustrations or photos can add into the richness of flow cytometry data. One example is, the determine demonstrates an usually typical apoptosis assay utilizing Annexin V and PI, adding cell imaging to characterize cells in Every inhabitants to reveal morphologically unique features. These insights couldn't are actually obtained from multiplex staining alone.
As samples are obtained within the Attune CytPix Flow Cytometer, the higher-speed brightfield digital camera captures and stores photographs of detected situations at a rate approximately 6,000 pictures for each second, based on the flow price and impression size. For bigger versatility, Attune Cytometric Program enables you to change the picture seize frequency as desired and choose a particular gate for image capture, if expected.
(B) RAW 264.7 macrophages are sometimes annotated inaccurately with respect to particle rely due to their complexity and noncircular borders. The quantity of singlets readily available for Evaluation boosts once the consumer trains and employs a far more accurate product.
Explore new insights into your experimental information by visualizing populations employing blended fluorescence and morphology data. Our software package interface wizards enable downsampling, concatenation (merged files) and dimensionality instruments that happen to be built into your application for an intuitive, streamlined facts analysis expertise.
"A picture just isn't quite truly worth a thousand dots, nonetheless it truly helps you to see illustrations or photos. It is great to discover illustrations or photos that correlate with staining. As an example, AnnexinV-positive cells that are morphologically unique than healthy cells, or CD14+ monocytes which can be much larger and more textured than CD3+/CD4+/CD14- lymphocytes.
Here, a highly skilled person has gated singlets confidently. Following analyzing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate consists of over 4% aggregates.
Pair your Attune flow cytometer with an autosampler for greater workflow efficiency, and incorporate robotic integration for max throughput.
Dependable impression high-quality even at superior throughput Acoustic focusing and also a higher-velocity camera Incorporate to picture these T cells regularly at lower or large flow costs. Conveniently regulate focus and digicam options to meet experimental prerequisites.
The Attune NxT cytometer is the responsible workhorse instrument your lab can trust in for consistent and trusted functionality. The Attune CytPix design includes a higher-pace brightfield digital camera and AI-driven impression Investigation software program, with latest upgrades such as machine Studying resources and an intuitive consumer interface, enabling Improved info precision, precision, and more rapidly insights into sample biology.
Consumers can check out celebration images in real-time to verify sample high quality, optimize protocols, and record check here the morphology of every cell population recognized for Examination. Imaging capture also Advantages from acoustic focusing, which really helps to situation the cells to ensure that a sharp, centered picture is obtained.
Audio waves help exact cell alignment, causing Extraordinary info high quality and ~10X more quickly sample flow rates.
We Earlier shown the power of imaging Vehicle-T/Ramos cell interactions. Enable’s look at just the population of best desire, the double beneficial activities, to learn more. We will now use prolonged morphological parameters (here, circularity and skewness of intensity) to more analyze the functions of those populations and refine gating on these gatherings, raising info robustness.
Leverage the strength of Superior technological innovation and innovation to speed up your discoveries. Trust during the Attune Flow Cytometer household to provide the dependability, velocity, and precision that set your get the job done apart.
Flexible payment program from Agilent Monetary Answers enable you to obtain the significant instrumentation you need for instant undertaking, or to develop your lab.
At the center of each and every Attune Flow Cytometer lies our groundbreaking acoustic focusing technologies, which makes use of sound waves to meticulously align cells throughout the flow mobile. This modern technique surpasses classic hydrodynamic methods, resulting in faster processing speeds, Fantastic knowledge top quality, and elevated sample throughput.
Since the cell line experienced Formerly been analyzed around the Attune CytPix Flow Cytometer, the researchers went back again to the images and had been in a position to document the microbial infection at the very least five days previously.
The Attune CytPix Flow Cytometer enables consumers to visualize samples all through acquisition, verify the accuracy of gating methods, and guidance success with responsible documentation for publication uses.
What seemed to be a homogeneous populace is definitely much more diverse—an insight that needs to be regarded as when interpreting benefits.