Knowledge-enriched characterization of cell populations. Morphological data from photos can insert towards the richness of flow cytometry details. For example, the figure displays an otherwise regular apoptosis assay making use of Annexin V and PI, incorporating cell imaging to characterize cells in Every populace to expose morphologically distinctive features. These insights couldn't happen to be acquired from multiplex staining alone.
As samples are acquired to the Attune CytPix Flow Cytometer, the large-pace brightfield camera captures and suppliers photos of detected functions in a amount around 6,000 images for each 2nd, dependant upon the flow amount and image size. For greater flexibility, Attune Cytometric Program enables you to regulate the impression capture frequency as wanted and select a particular gate for impression seize, if expected.
Incorporate acoustic concentrating know-how with higher-parameter Examination for versatile spectral unmixing and conventional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating by using a substantial-pace brightfield camera for simultaneous large throughput flow cytometry and large resolution brightfield imaging.
"An image just isn't very worthy of one thousand dots, nevertheless it definitely really helps to see photographs. It is excellent to check out illustrations or photos that correlate with staining. For instance, AnnexinV-positive cells which can be morphologically unique than balanced cells, or CD14+ monocytes which can be much larger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it really is illuminating to discover the amount of doublets slip by standard singlet gates, and excellent to confirm the position of unpredicted double-optimistic gatherings in my Evaluation."
Optimizing gating approach. Even sturdy manual singlet gating is error-inclined and stays a subjective final decision issue in Virtually all flow cytometry assays. Imaging can be utilized to confirm and adjust gates to include only solitary cells of curiosity.
Steady impression quality even at substantial throughput Acoustic concentrating along with a large-velocity camera Incorporate to impression these T cells continuously at reduced or substantial flow prices. Simply alter concentration and digital camera settings to fulfill experimental prerequisites.
Early indications of this contamination had initially been dismissed as particles. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also means that you can use morphological attributes to discover interesting subpopulations that may not be apparent from flow cytometry knowledge by itself.
Probably most importantly, these events include cells of Obviously distinctive phenotypes which may lead to incorrect conclusions pertaining to double constructive activities (especially in exceptional populations).
AI-driven automated impression analysis with the Attune Cytometric Software program translates celebration capabilities into unique morphology parameters which might be coupled with regular fluorescence and scatter parameters to expose very reproducible population improvements and one of a kind subsets.
Application Take note: Automated image Investigation minimizes user-to-person variability in flow cytometry gating strategies
Leverage the power of State-of-the-art technologies and innovation to accelerate your discoveries. Rely on from the Attune Flow Cytometer spouse and children to provide the dependability, velocity, and precision that set your operate apart.
Versatile payment approach from Agilent Economical Options assist you to obtain the important instrumentation you need for immediate undertaking, or to develop your lab.
At the center of each and every Attune Flow Cytometer lies our innovative acoustic concentrating know-how, which makes use of audio waves to meticulously align cells throughout the flow cell. This modern tactic surpasses standard hydrodynamic strategies, leading to quicker processing speeds, Extraordinary facts high-quality, and increased sample throughput.
The Attune Xenith cytometer is usually a higher-parameter spectral analyzer, offering equally spectral unmixing and conventional flow more info capabilities for complete and versatile cell Examination.
Agilent Benefit Assure assures you not less than ten years of instrument use in the day you buy. If not, we will credit rating you While using the residual value of the technique toward an upgraded model.
Here, we exhibit that by gating approaches utilizing image-primarily based quantitative parameters can distinguish interacting cells from coincident functions to a lot more properly examine interacting cells.